HIGH-YIELD PREPARATION OF ISOLATED RAT LIVER PARENCHYMAL CELLS
نویسندگان
چکیده
منابع مشابه
High-yield Preparation of Isolated Rat Liver Parenchymal Cells
A new technique employing continuous recirculating perfusion of the rat liver in situ, shaking of the liver in buffer in vitro, and filtration of the tissue through nylon mesh, results in the conversion of about 50% of the liver into intact, isolated parenchymal cells. The perfusion media consist of: (a) calcium-free Hanks' solution containing 0.05% collagenase and 0.10% hyaluronidase, and (b) ...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1969
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.43.3.506